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Image Search Results
Journal: PLoS ONE
Article Title: Attenuation of Vaccinia Tian Tan Strain by Removal of Viral TC7L-TK2L and TA35R Genes
doi: 10.1371/journal.pone.0031979
Figure Lengend Snippet: Effects of MVTT1, MVTT2, MVTT3, or VTT (0.05 PFU/cell) and infection times on the cell viabilities of HeLa (A), MDCK (B), PK(15) (C), BHK-21 (D), and Vero (E). Cells were seeded in 96-well plates (1×10 4 cells/well) one day before they were infected with 0.05 PFU/cell of MVTT1, MVTT2, MVTT3, or VTT. Cell viability was measured daily for 4 d by MTT colorimetric assay. All measurements were performed in triplicate. Data are presented as mean ± SD.
Article Snippet: BHK-21 hamster kidney cells, PK(15) porcine kidney cells, HeLa human cervical adenocarcinoma epithelial cells, Madin-Darby canine kidney (MDCK) epithelial cells, and
Techniques: Infection, Colorimetric Assay
Journal: PLoS ONE
Article Title: Attenuation of Vaccinia Tian Tan Strain by Removal of Viral TC7L-TK2L and TA35R Genes
doi: 10.1371/journal.pone.0031979
Figure Lengend Snippet: Confluent monolayers of PK(15), MDCK, HeLa, BHK-21 and Vero cells in 12-well plates were infected with 0.05 PFU/cell of MVTT1, MVTT2, MVTT3, or VTT viruses. The plates were incubated at 37°C for 2 d prior to staining with 0.1% crystal violet. The pathogenicity of MVTT1, MVTT2, and MVTT3 apparently decreased in all five cell lines (compared with VTT as the control).
Article Snippet: BHK-21 hamster kidney cells, PK(15) porcine kidney cells, HeLa human cervical adenocarcinoma epithelial cells, Madin-Darby canine kidney (MDCK) epithelial cells, and
Techniques: Infection, Incubation, Staining, Control
Journal: PLoS ONE
Article Title: Attenuation of Vaccinia Tian Tan Strain by Removal of Viral TC7L-TK2L and TA35R Genes
doi: 10.1371/journal.pone.0031979
Figure Lengend Snippet: PK(15), MDCK, HeLa, BHK-21 and Vero cells infected with 0.05 MOI of VTT and the mutants, and then the viruses were harvested and titrated in BHK-21 cells. Virus titer was determined by measuring the plaque assays.
Article Snippet: BHK-21 hamster kidney cells, PK(15) porcine kidney cells, HeLa human cervical adenocarcinoma epithelial cells, Madin-Darby canine kidney (MDCK) epithelial cells, and
Techniques: Infection, Virus